expression vector encoding cas9 2a gfp (Addgene inc)
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expression vector encoding cas9 2a gfp
Expression Vector Encoding Cas9 2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+encoding+cas9+2a+gfp/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm35963869-288-38-43
Average 96 stars, based on 4084 article reviews
Expression Vector Encoding Cas9 2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4084 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+vector+encoding+cas9+2a+gfp/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm35963869-288-38-43
Average 96 stars, based on 4084 article reviews
expression vector encoding cas9 2a gfp - by Bioz Stars,
2026-09
96/100 stars
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Expressing:Article Title: XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair. Article Snippet: .. To generate knockouts, U2OS (FRT) cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/SigmaAldrich sgRNA library (see Supplementary Table 3 for plasmids, Supplementary Table 4 for sgRNA sequences) targeting a specific gene together with an Article Title: Human HMGN1 and HMGN2 are not required for transcription-coupled DNA repair Article Snippet: .. To generate single knockouts, U2OS (FRT) cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/Sigma-Aldrich sgRNA library (see for plasmids, for sgRNA sequences) targeting the HMGN1, CSB or XPA gene together with an Article Title: Transcription-coupled nucleotide excision repair is coordinated by ubiquitin and SUMO in response to ultraviolet irradiation Article Snippet: U2OS Flp-In/T-REx cells, which were generated using the Flp-InTM/T-RExTM system (Thermo Fisher Scientific), were a gift of Daniel Durocher. .. These cells were co-transfected with pLV-U6g-PPB containing an antisense guide RNA targeting the CSA/ERCC8 gene (5'-CCAGACTTCAAGTCACAAAGTTG-3') from the Sigma-Aldrich sgRNA library together with an Article Title: ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination Article Snippet: Single clones were selected by limited dilution, and protein levels were screened by western blotting (WB) and immunofluorescence (IF) using ZMYM2 antibodies (Bethyl Laboratories #A301-709A 1:1000, Abcam #ab106624 1:1000, and Sigma #HPA031765 1:100, respectively). .. To generate XPC knockouts, U2OS(FRT) cells were co-transfected with pLV-U6g-PPB encoding an XPC guide RNA from the LUMC / Sigma-Aldrich sgRNA library (5-TGGGGGTTTCTCATCTTCAAAGG-3) together with an Article Title: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II. Article Snippet: .. To generate stable knockouts, U2OS Flp-In/TREx cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/Sigma-Aldrich sgRNA library (see Supplementary Table 2 for plasmids, Supplementary Table 3 for sgRNA sequences) together with an Article Title: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II Article Snippet: U2OS Flp-In/T-REx cells, which were generated using the Flp-In/T-REx system (Thermo Fisher Scientific), were a gift from Daniel Durocher . .. To generate stable knockouts, U2OS Flp-In/T-REx cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/Sigma-Aldrich sgRNA library (see Supplementary Table for plasmids, Supplementary Table for sgRNA sequences) together with an Article Title: ERCC1 mutations impede DNA damage repair and cause liver and kidney dysfunction in patients Article Snippet: .. To generate single KOs, U2OS(FRT) and RPE1-hTERT (PuroR/TP53-dKO) cells were cotransfected with pLV-U6g-PPB encoding a guide RNA from the Leiden University Medical Center/Sigma-Aldrich single-guide RNA (sgRNA) library (see for plasmids and for sgRNA sequences) targeting the ERCC1 gene together with an Article Title: XPC–PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair Article Snippet: .. To generate knockouts, U2OS (FRT) cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/Sigma-Aldrich sgRNA library (see Supplementary Table for plasmids, Supplementary Table for sgRNA sequences) targeting a specific gene together with an Plasmid Preparation:Article Title: XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair. Article Snippet: .. To generate knockouts, U2OS (FRT) cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/SigmaAldrich sgRNA library (see Supplementary Table 3 for plasmids, Supplementary Table 4 for sgRNA sequences) targeting a specific gene together with an Article Title: Human HMGN1 and HMGN2 are not required for transcription-coupled DNA repair Article Snippet: .. To generate single knockouts, U2OS (FRT) cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/Sigma-Aldrich sgRNA library (see for plasmids, for sgRNA sequences) targeting the HMGN1, CSB or XPA gene together with an Article Title: Transcription-coupled nucleotide excision repair is coordinated by ubiquitin and SUMO in response to ultraviolet irradiation Article Snippet: U2OS Flp-In/T-REx cells, which were generated using the Flp-InTM/T-RExTM system (Thermo Fisher Scientific), were a gift of Daniel Durocher. .. These cells were co-transfected with pLV-U6g-PPB containing an antisense guide RNA targeting the CSA/ERCC8 gene (5'-CCAGACTTCAAGTCACAAAGTTG-3') from the Sigma-Aldrich sgRNA library together with an Article Title: ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination Article Snippet: Single clones were selected by limited dilution, and protein levels were screened by western blotting (WB) and immunofluorescence (IF) using ZMYM2 antibodies (Bethyl Laboratories #A301-709A 1:1000, Abcam #ab106624 1:1000, and Sigma #HPA031765 1:100, respectively). .. To generate XPC knockouts, U2OS(FRT) cells were co-transfected with pLV-U6g-PPB encoding an XPC guide RNA from the LUMC / Sigma-Aldrich sgRNA library (5-TGGGGGTTTCTCATCTTCAAAGG-3) together with an Article Title: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II. Article Snippet: .. To generate stable knockouts, U2OS Flp-In/TREx cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/Sigma-Aldrich sgRNA library (see Supplementary Table 2 for plasmids, Supplementary Table 3 for sgRNA sequences) together with an Article Title: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II Article Snippet: U2OS Flp-In/T-REx cells, which were generated using the Flp-In/T-REx system (Thermo Fisher Scientific), were a gift from Daniel Durocher . .. To generate stable knockouts, U2OS Flp-In/T-REx cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/Sigma-Aldrich sgRNA library (see Supplementary Table for plasmids, Supplementary Table for sgRNA sequences) together with an Article Title: ERCC1 mutations impede DNA damage repair and cause liver and kidney dysfunction in patients Article Snippet: .. To generate single KOs, U2OS(FRT) and RPE1-hTERT (PuroR/TP53-dKO) cells were cotransfected with pLV-U6g-PPB encoding a guide RNA from the Leiden University Medical Center/Sigma-Aldrich single-guide RNA (sgRNA) library (see for plasmids and for sgRNA sequences) targeting the ERCC1 gene together with an Article Title: XPC–PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair Article Snippet: .. To generate knockouts, U2OS (FRT) cells were co-transfected with pLV-U6g-PPB encoding a guide RNA from the LUMC/Sigma-Aldrich sgRNA library (see Supplementary Table for plasmids, Supplementary Table for sgRNA sequences) targeting a specific gene together with an |